Innovative, Sustainable and inclusive Bioeconomy
H2020-ISIB-2015-1Sub call of: H2020-ISIB-2014-2015
| Publication date | 2013-12-11 | Deadline Date | 2015-06-11 17:00:00 (Brussels local time) |
| Total Call Budget | €25,000,000 | Main Pillar | Societal Challenges |
| Status | Open | OJ reference | OJ C361 of 11 December 2013 |
Topic: Biomarkers for nutrition and health
ISIB-12f-2015
Combined group of experts included researches from Biological-Chemical Department of University of Bialystok, Technical University of Bialystok is looking for partners and coordinatinginstitution for the implementation of the following project: Studies on the correlation between the structure and activity of molecular electron transition metal complexes with ligands of biological importance.
We’ll welcome any proposal for cooperation and participation in research projects, coming from other colleagues.
Contact person: prof. Joanna Karpinska, e-mail: joasia@uwb.edu.pl
Main goal of project: Finding new biologically active compounds with potential use in the pharmaceutical and/or food industry.
Description of project: Plant’s originated drugs in the form of decoctions, extracts, infusions accompanied mankind since the dawn of history. Systematic studies confirmed their pharmacological activity allow specification of the active compound. All the possibilities of the plant world are still not recognized. The investigation of compounds of natural origin is a difficult task requiring the involvement of a wide range of experts beginning from analytical chemists, through spectral analysis specialists and organic chemists ending. An obtaining of active ingredient from the plant material is often difficult due to the presence of trace amounts or of its low durability.
Our project involves the study of chemical-biological activity of plant-derived ligands such as curcumin and some active compounds from tannin group of compounds like tannin acid and 3,6-bis-О-di-О-galoilo-1,2,4-tri-О-galoilo-β-D- glucose.
The following activities are planning:
-synthesis of chemically modified molecules of examined ligands (e.g. dimethoxycurcumin, bidimethoxy-curcumin, tropane analogues of curcumin) and examination of their stability, permeability through biological membranes and spectral characteristics;
– Synthesis of connections of studied ligands with metal ions: Ru(II), Ru(III), Os(II), Pd(II), Pd(IV), Pt(II), Pt(IV), Ag(I), Au(I), Au(III) and examination of their stability, permeability through biological membranes, spectral characteristics in correlation of their electronic structure;
– formation of conjugates: antibiotic-ligand, ligand-nanoparticle, nanoparticle-ligand- monoclonal antibody and the study of their stability, the electron distribution, permeability through biological membranes;
– molecular modeling to determine an optimal structure of the investigated ligands, their complexes and conjugates;
-studies in vitro on biological activity of received conjugates and compounds using cells lines fibroblasts, HeLa, HepG2, and others). The planned analysis will include:
a) cytotoxicity tests in relation to the selected cell lines ,
b) interaction with the cell membrane (analysis of changes in membrane fluidity, the membrane potential),
c) measurement of caspase activity,
d) study onselectedsignaling pathwaysin the cell
e) examination of the position of the membrane phosphatidylserine.
– studies in vivo on biological activity of received conjugates and compounds:
a) examination of genotoxicity (e.g. Ames test, test thymidine kinase gene mutation mouse lymphoma, micronucleus test),
b) studies on laboratory organisms,
c) examination antioxidative/prooxidative activity of received compounds and conjugates.
The studies of molecular structure, the charge distribution of the electron and the physicochemical properties of the synthesized compounds will be performed using:
a) infrared spectroscopy (FT-IR) in solid phase and in solution,
b) Raman spectroscopy (FT-Raman),
c) UV-Vis- NIR spectroscopy,
d) Spectrofluorimetric technique,
e) Nuclear magnetic resonance spectroscopy (1H, 13C i 15N NMR),
f) Single crystalX-ray diffraction,
g) Elemental andthermogravimetric analysis.
There is expected that the conducted studies allows selecting new types of modification of the above-mentioned compounds, which are to ensure a high biological activity in ratio to selected cell lines. Such modified compounds with proven high activity will be the basis for the development of new drugs in anticancer therapies. In addition, we expect that our research will help to identify new local sources of biologically active compounds, and thus may affect the development of the local economy.
